T developments in ionization methods which includes MALDI-2 and their application as an illustration in redox lipidomics, give an exciting opportunity to study uncommon lipids [726, 727]. In view in the ongoing shift in cancer study towards single cell approaches revealing the importance of Complement Component 1 Proteins custom synthesis tissue heterogeneity in cancer progression and therapeutic outcome, we’ll witness a shift within the lipidomics field from lipidomics analyses on bulk tissue to single cell and spatial evaluation by mass spectrometry imaging. New technological developments within this domain guarantee an unseen functionality with regards to analytical aspects also as spatial resolution, top to novel insights inside the part of lipids in the complicated metabolic interplay between unique cell types within the heterogenous tumor microenvironment. With novel technologies enabling imaging of lipids at the intracellular level such as dynamic SIMS ion microcopy and Raman microscopy, a whole new location of lipid analysis is opening up, revealing adjustments in organellar lipidomes, trafficking pathways and membrane structures [728, 729]. The additional improvement of stable isotope lipidomics will permit to adhere to alterations in pathway fluxes rather of current steady state evaluation and with each other with spatial multi-omics approaches will give unprecedented insight in affected pathways and prospective biomarkers. In addition to these developments there is an urgent will need for standardization of procedures and technologies to enable future clinical implementations of your found biomarkers. When it comes to therapeutic prospective, current findings recommend that interference with lipid metabolism may have promising applications, especially in combinatorial approaches[16]. With small molecules targeting enzymes in lipid metabolism getting into clinical trials we are at the doorstep of witnessing the clinical exploitation of altered lipid metabolism as a hallmark of cancer. The hyperlink using the diet regime, such as dietary lipids will also build exceptional possibilities for preventive techniques and therapy enhancement. Specifically inside the field of tumor immunology, lipids hold fantastic prospective as modulators. In summary, although lagging behind in comparison to other omics approaches, the study of lipids in cancer is rapidly catching up and is establishing Stimulatory immune checkpoint molecules Proteins Purity & Documentation itself as a central hallmark of cancer with promising opportunities for clinical application.Author Manuscript Author Manuscript Author Manuscript Funding Author ManuscriptThis function was supported by an EU Interreg grant V-A EMR23 EURLIPIDS, KU Leuven grants C1 (C16/15/073) and C3 (C32/17/052), Investigation Foundation-Flanders (FWO), Stichting tegen Kanker, Kom op tegen Kanker, the Movember Foundation/Prostate Cancer Foundation of Australia (MRTA3), The Prostate Cancer Foundation of Australia (ID NDDA), the Cancer Council South Australia Beat Cancer Project, NIH grant RO1CA58961, a Norris Cotton Cancer Center grant, and the Dartmouth College Norris Cotton Cancer Center Support Grant P30CA023108. ML’s function is supported by NIH grants RO1CA131945, R01CA187918, DoD PC160357, DoD PC180582, P50CA211024, as well as the Prostate Cancer Foundation.AbbreviationsAA ACACA arachidonic acid acetyl-CoA carboxylase alphaAdv Drug Deliv Rev. Author manuscript; obtainable in PMC 2021 July 23.Butler et al.PageACACBacetyl-CoA carboxylase beta ATP-citrate lyase Acyl-CoA Synthetase acylprotein thioesterase androgen receptor acid sphingomyelinase adipose triglyceride lipase autotaxin breast cancer clear cell renal cell carcinoma chol.