Nt of Anesthesiology and Program in Neuroscience, College of Medicine, University of California, San Diego, CA, USAIntroduction: Angiogenesis plays a essential function in tissue repair. This procedure is substantially impaired by age-related dysfunction of vascular endothelial cells in aged bodies. Exosomes from embryonic stem cells (ESCs) contain primitive molecules (proteins, miRNA, and so forth.) from their parent cells. Hence, our hypothesis is that ESCs derived exosomes (ES-Exos) would influence and rejuvenate aging endothelial cells and result in enhanced tissue repair in aged bodies. Procedures: Six- to eight-week-old C57BL/6 mice had been every day subcutaneous injection of D-gal (1000 mg/kg) to establish aged mice model. Stress ulcers have been developed on the back of each mouse, followed by pipetting ESExos (11011/mL) suspension or PBS one time every day. Mice have been sacrificed at 3, 7, 14, and 21 days soon after intervention. In addition, a group of young mice with stress ulcer was also set. Samples from each mouse had been evaluated inside the aspect of vascular formation and aging condition. Moreover, we induced HUVEC senescence in vitro by D-gal treatment and investigate the function and mechanism of Gastrin Proteins Formulation ES-Exos in restoring function and rejuvenation of senescent endothelial cells by qRT-PCR, WB, and immunofluorescent staining. Final results: Our final results showed that ES-Exos treated aged mice exhibit faster repairing than PBS treated group. The angiogenesis situation of ES-Exos treated group was related as that of young mice and was better than PBS treated senescent mice. The amount of SA–galpositive cells and the expression degree of P16 and P21 in ES-Exos treated group had been drastically decrease than that in PBS treated group. In vtiro experiments showed that ES-Exos could also downregulate senescent related protein Siglec-5/CD170 Proteins Molecular Weight expressions and improve tube formation of senescent endothelial cells. Additionally, our benefits also showed that ES-Exos could greatly decreased the expression amount of MDA and raise the activity of SAD, CAD, and GSH, molecules tightly related with endogenous anti-oxidative condition. Further investigation demonstrated that ES-Exos could activate NRf2 pathway by inhibiting Keap1, major to rejuvenative function on senescent endothelial cells. Summary/Conclusion: We demonstrate that ES-Exos can accelerate wound healing and market angiogenesis in aged mice by rejuvenating endothelial senescence. Funding: NSFC Project No. 81871833 and 81672254.OF17.Schwann cell derived exosomes regulate Schwann cell activation and neuropathic discomfort connected behaviours Naoya Hirosawaa, HyoJun Kwonb, Haylie Romerob, John Kimb, Coralie Brifaultc, Seiji Ohtorid and Wendy CampanaeIntroduction: Exosomes (Exs) are smaller extracellular vesicles originally recognized to be secreted from multivesicular endosomes in dendritic cells. We now know that Exs are secreted from a lot of cell kinds and are vital for autocrine/paracrine communication. Inside the peripheral nervous program (PNS), Exs derived from major Schwann cells (SC) appear to facilitate axon development following injury, having said that their effects on SC physiology and discomfort outcomes are unknown. Approaches: Exs have been purified from key SC conditioned media by ultracentrifugation (SC-Ex) and characterized by immunoblotting and NanoSight In cultures of SC, TNFa robustly activated proinflammatory cell signalling and migration. SC-Ex (5000 ng/ mL) had been added to TNFa treated SC, and phosphorylation of p38MAPK and JNK1/2 have been measured. Transwells were applied to.