Product Name :
Anti-Goat IgG(H+L), Rabbit antibody(iFluor647)
Applications:
ELISA,WB,ICC/IF,Flow Cyt
Reactivity :
Goat IgG(H+L)
Conjugate:
iFluor647
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Goat IgG(H+L), Rabbit antibody(iFluor647) is designed for detecting goat IgG(H+L) specifically. Anti-Goat IgG(H+L), Rabbit antibody(iFluor647) is based on recombinant, rabbit IgG Fc fused single domain antibody to goat IgG(H+L) coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Goat IgG(H+L), Rabbit antibody(iFluor647) reacts with goat IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, rabbit IgG. | Immunogen: Recombinant goat IgG | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation) | Conjugate: iFluor647(Ex: 652nm, Em: 668nm), 3 moles iFluor647 per mole IgG | Specificity: Goat IgG(H+L) | Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, rabbit IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS(pH 7.{{366-93-8} site|{366-93-8} Biological Activity|{366-93-8} Purity|{366-93-8} manufacturer} 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol | Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light | Background:Goat antibodies are commonly used in biotechnology. They are used to prepare diagnostic reagents of immunochemical techniques. Goat IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kDa.Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-Goat IgG(H+L), Rabbit antibody(iFluor647) is designed for detecting goat IgG(H+L) specifically. Anti-Goat IgG(H+L), Rabbit antibody(iFluor647) is based on recombinant, rabbit IgG Fc fused single domain antibody to goat IgG(H+L) coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Goat IgG(H+L), Rabbit antibody(iFluor647) reacts with goat IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, rabbit IgG.
Immunogen:
Recombinant goat IgG
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), Protect from light
Function:
Flow Cyt: 1:200-1:2000ICC/IF： 1:200-1:2000ELISA: 1:5000 -1:20000WB: 1:5000 -1:20000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc.{{1441190-66-4} site|{1441190-66-4} Biological Activity|{1441190-66-4} Purity|{1441190-66-4} custom synthesis} The actual dilution used must be determined empirically.PMID:30725923