Product Name :
Anti-Myc tag, Rabbit antibody(iFluor594)
Applications:
WB,ICC/IF,ELISA,Flow Cyt
Reactivity :
Myc tag
Conjugate:
iFluor594
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Myc tag, Rabbit antibody(iFluor594) is designed for detecting Myc tag fusion proteins specifically. Anti-Myc tag, Rabbit antibody(iFluor594) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 antibody to Myc tag coupled to iFluor594, and Anti-Myc tag, Rabbit antibody(iFluor594) detects the Myc tag selectively, no reactivity with other proteins.{{404951-53-7} MedChemExpress|{404951-53-7} Protocol|{404951-53-7} Formula|{404951-53-7} supplier} | Immunogen: Myc tag fusion protein | Host: Alpaca pacous | Isotype: Fab of alpaca IgG1 fused to Rabbit Fc(mutation) | Conjugate: iFluor594(Ex: 592nm, Em: 614nm), 3 moles iFluor594 per mole IgG | Specificity: Myc tag sequence(EQKLISEEDL) | Cross-Reactivity: Highly selective for Myc tag sequence | Purity: Recombinant expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol | Storage: Store at –20 °C, (Avoid freeze / thaw cycles), protect from light | Background:The Myc peptide are widely used for detecting or manipulating proteins.{{2378407-27-1} web|{2378407-27-1} Biological Activity|{2378407-27-1} In stock|{2378407-27-1} manufacturer} This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.PMID:29939606 Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-Myc tag, Rabbit antibody(iFluor594) is designed for detecting Myc tag fusion proteins specifically. Anti-Myc tag, Rabbit antibody(iFluor594) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 antibody to Myc tag coupled to iFluor594, and Anti-Myc tag, Rabbit antibody(iFluor594) detects the Myc tag selectively, no reactivity with other proteins.
Immunogen:
Myc tag fusion protein
Host :
Alpaca pacous
Isotype:
Fab of alpaca IgG1 fused to Rabbit Fc(mutation)
Purity :
Recombinant expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, (Avoid freeze / thaw cycles), protect from light
Function:
WB: 1:5000 -1:20000ELISA: 1:5000 -1:20000Flow Cyt: 1:200-1:2000ICC/IF: 1:200-1:2000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.