Product Name :
TRFS-green
Description:
TRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm.
CAS:
1513848-14-0
Molecular Weight:
416.51
Formula:
C20H20N2O4S2
Chemical Name:
1,2-dithiolan-4-yl N-{3-butyl-2,4-dioxo-3-azatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaen-8-yl}carbamate
Smiles :
CCCCN1C(=O)C2C=CC=C3C=2C(=CC=C3NC(=O)OC2CSSC2)C1=O
InChiKey:
MUAYCZWBPDUZKR-UHFFFAOYSA-N
InChi :
InChI=1S/C20H20N2O4S2/c1-2-3-9-22-18(23)14-6-4-5-13-16(8-7-15(17(13)14)19(22)24)21-20(25)26-12-10-27-28-11-12/h4-8,12H,2-3,9-11H2,1H3,(H,21,25)
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life:
≥12 months if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
TRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm.|Product information|CAS Number: 1513848-14-0|Molecular Weight: 416.51|Formula: C20H20N2O4S2|Chemical Name: 1,2-dithiolan-4-yl N-{3-butyl-2,4-dioxo-3-azatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaen-8-yl}carbamate|Smiles: CCCCN1C(=O)C2C=CC=C3C=2C(=CC=C3NC(=O)OC2CSSC2)C1=O|InChiKey: MUAYCZWBPDUZKR-UHFFFAOYSA-N|InChi: InChI=1S/C20H20N2O4S2/c1-2-3-9-22-18(23)14-6-4-5-13-16(8-7-15(17(13)14)19(22)24)21-20(25)26-12-10-27-28-11-12/h4-8,12H,2-3,9-11H2,1H3,(H,21,25)|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.Crystal Violet |Shelf Life: ≥12 months if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|TRFS-green itself emits at ∼480 nm when excited at 377 nm. TRFS-green displays a green fluorescence off−on change induced by the TrxR-mediated disulfide cleavage and subsequent intramolecular cyclization to liberate the masked naphthalimide fluorophore.DCVC The fluorescence signal of TRFS-green in living cells was inhibited by pretreating the cells with TrxR inhibitor in a dose-dependent manner, potentiating the development of living cell-based screening assay for identifying TrxR inhibitors.PMID:27108903 After incubating the cells with TRFS-green (10 μM) for 4 h, the bright green fluorescence appears. TRFS-green shows no response to GSH or Cys, the predominant thiols in cells, or the endogenous reducing agents, such as vitamin C and NADPH. TRFS-green is the first fluorescent probe of TrxR with a green emission. However, the response rate of TRFS-green to the enzyme is slow, and it takes more than 2 h to reach the maximal fluorescence signal even using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent.|Products are for research use only. Not for human use.|