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Opioid receptor κ polyclonal antibody
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The opioid receptors delta (DOP, OP1), kappa (KOP, OP2), and mu (MOP, OP3) are members of the seven-transmembrane domain-containing receptor family of G-protein coupled receptors, sharing nearly 60% identity, but varying greatly in the extracellular N-terminus region of the receptors. The delta, kappa and mu-receptors serve as functional receptors for endogenous enkephalin, dynorphin, and beta-endorphin peptide ligands, respectively, mediating their signal via coupling to Gi/o alpha subunits to inhibit adenylyl cyclases and calcium conductance (N- and L-type channels), and to stimulate MAPK activation and potassium conductance. Delta-opioid receptors are highly expressed in the olfactory bulb, neocortex, caudate putamen and nucleus accumbens, with peripheral expression in the gastro-intestinal tract and vas deferens. Delta-opioid receptors function to inhibit neurotransmitter release resulting in a variety of biological effects including analgesia, motor integration, gastro-intestinal motility, olfaction, Western blot analysis: MW marker (1) and 50µg rat brain extract (1) probed with Opioid Receptor kappa pAb at 1µg/ml Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 μM concentrations of agonist (Endorphin B) and probed with Opioid Receptor kappa pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4). Western blot analysis: MW marker (1) and 50µg rat brain extract (1) probed with Opioid Receptor kappa pAb at 1µg/ml Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 μM concentrations of agonist (Endorphin B) and probed with Opioid Receptor kappa pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA.{{168021-79-2} web|{168021-79-2} Protocol|{168021-79-2} Description|{168021-79-2} manufacturer} Data from vehicle treated cells were taken as 100%.{{28097-03-2} site|{28097-03-2} Biological Activity|{28097-03-2} In stock|{28097-03-2} supplier} Results are the mean ± SEM (n=4).PMID:22319799
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| Alternative Name OPRK1, KOR-1 | Application ELISA, IHC, WB | Application Notes Suitable for cell-based and membrane-based ELISA.Predicted MW of ~43kDa. Higher MW species (~50kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor. | Crossreactivity Predicted species reactivity based on epitope sequence homology: dog, bovine, horse. | Formulation Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. | Host Rabbit | Immunogen Synthetic peptide corresponding to the sequence near the N-terminus of mouse opioid receptor κ. | Species Reactivity Human, Mouse, Rat | UniProt ID P33534 | Unit of Measure (UM) µg