Arrowheads show the dot-like structures of GFP-Syb1 and the Golgi/endosomes stained with FM4-sixty four, double arrowheads show cytoplasmic accumulation, and arrows indicate the concentrated fluorescence at the medial region and cell surface area
Next, we assessed whether several varieties of Rho3 are associated with Sip1. For this goal, wild-form Rho3, the nucleotide-locked forms of Rho3 and a Rho3E48V effector area mutant had been…