the microarrays were washed and scanned with the Agilent Microarray Scanner G2505C in a solitary pass mode with a scan resolution of 3 m, 20 bit method
Quadruplicate samples of murine skeletal myoblasts, differentiated myotubes, and TNF- or IGF1 handled myotubes had been profiled. We employed one hundred ng overall RNA for Cy3-labeling of miRNAs by utilizing…