To examination whether or not TAR current on transcripts synthesized by the replicating virus can act equally to suppress silencing, we transfected HEK-293T cells with the HIV-one infectious molecular clone, pLAI, and assessed silencing of EGFP reporter by a cotransfected miEGFP expression plasmid
Even though Tat overexpression does not impact silencing by either exogenous or endogenous miRNAs, it remained possible that other viral proteins or RNAs could alter the efficacy of RNAi. For…