Membranes have been then incubated for one hour with secondary antibodies, conjugated with horseradish peroxidase. Proteins have been detected through the addition of ECL reagent (Pierce) as a substrate and exposed to an autoradiographic film (Amersham)
Two distinctive human breast cancer cell traces have been employed in this research: MCF7/AZ had been stably transduced with vacant vector or CDH3/ Pcadherin cDNA, respectively] and SUM149 . The…