The resins are washed extensively with buffer B, and bound proteins have been eluted with buffer B that contains two hundred mM imidazole
b2AR was reconstituted into rHDL as formerly explained . Briefly, a mixture of palmitoyl-oleoyl-glycero-phosphocholine and palmitoyl-oleoyl-phosphatidylglycerol ended up utilised in mixture (three:two molar ratio) to mimic the zwitterionic surroundings of…